PCR for capsular typing of Haemophilus influenzae
نویسندگان
چکیده
منابع مشابه
Real-time PCR for determining capsular serotypes of Haemophilus influenzae.
A two-step real-time PCR assay targeting all six capsulation loci of Haemophilus influenzae (i.e., serotypes a to f) was developed and compared with a previously published qualitative PCR assay by using 131 H. influenzae clinical isolates. There was a 98.5% concordance between the two tests. The sensitivity of detection of capsular type-specific reference strains of H. influenzae a to c (10(1) ...
متن کاملBiotyping, capsular typing, and antibiotic resistance pattern of Haemophilus influenzae strains in Iran.
The aim of this study was to determine the capsular types of Haemophilus influenzae isolated from clinical specimens by slide agglutination serotyping (SAST) and PCR capsule typing methods. All the isolates were biotyped and their antibiotic resistance patterns also determined. Thirteen isolates of serotype b, 2 of serotype e, 4 of serotype f, and 19 nontypeable (NT) isolates were identified by...
متن کاملRibotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil.
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of...
متن کاملMeasurement of Antibodies Reacting with Capsular Antigens of Haemophilus Influenzae.
A simple haemagglutination procedure is described which measures antibodies that react with capsular antigens of Haemophilus influenzae. Abnormally high antibody titres of appropriate type-specificity are common in the blood of human subjects who are known to have carried capsulate strains of H. influenzae. It is not yet clear whether all such antibodies are due to such carriage.
متن کاملHaemophilus Influenzae B
یکی از شایع ترین مننژیت باکتریایی در شیرخواران، هموفیلوس آنفلوانزا از نوع B (HIB) می باشد. به منظور تهیه و عرضه واکسنی جهت حفظ کودکان در مقابل این بیماری به بازار جهانی، تحقیقاتی انجام و نوعی واکسن در بخش تحقیقاتی آکسفورد مورد آزمایش و ارزیابی قرار گرفت تا در صورت تمایل والدین و کارکنان بهداشتی، تجویز همزمان آن با واکسن سه گانه و فلج اطفال عملی گردد.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.10.2382-2386.1994